Abstract: The biomorphic MnO2 materials with lamellar porous structure were successfully synthesized by using camellia petal as biotemplate and MnNO3 solution of 0.01,0.03,0.05,0.10 mol·L-1 as precursor. Their structure and morphology were characterized. And then lithium-ion battery was assembled by using MnO2 as negative electrode material, the electrochemical properties of MnO2 was tested. Results show that all MnO2 contained α, β and ε form MnO2 crystals. The specific surface area and pore volume of MnO2 respectively increased from 8.81 cm2·g-1 to 39.82 cm2·g-1 and from 0.08 cm3·g-1 to 0.26 cm3·g-1 with the concentration of precursor solution increased from 0.01 mol·L-1 to 0.10 mol·L-1. In addition, the first coulombic efficiency of lithium-ion battery was more than 22.56%, and the reversible capacity of the battery could still maintain 70 mAh·g-1 after 10 cycles.