Abstract: Sol-gel method and co-precipitation method were combined to prepare bioactive glass (BAG) wet get with different amounts of silicon, and the wet get was dried at vacuum freeze-drying and vacuum heat-drying modes to prepare spherical BAG powders (58S powders, 68 powders and 77S powders). The effects of dispersant PEG (polyethylene glytol), silicon content and drying modes on the size and morphology of BAG powders were studied. And then, mineralization test was carried out by adding 68S powders to simulated body fluid (SBF). The results show that PEG can control effectively the shape and size of BAG powders, and make the particles keep sphericity. 68S powders has the smallest size, the most smooth surface and the best degree of sphericity. Vaccum freeze-drying can effectively restraine the aggregation of wet get during drying. Carbonate hydroxyl apatite(HCA) was formed on BAG powder surface after mineralization, which made BAG powders have good biological activity.