Abstract: High-temperature compression tests were carried out on Ti6Al4V alloy prepared by powder hot isostatic pressing with a Gleeble3500 thermal simulator at different temperatures and strain rates. A constitutive equation describing the compression behavior of the alloy in two-phase region was established. The hardening, dynamic softening parameters, and dynamic recrystallization kinetics models of the alloy during hot working were solved. The flow stress model of the alloy in two-phase region was constructed. The results show that the microstructure of the prepared Ti6Al4V alloy was composed of α phase and β phase and presented a typical grid structure. The grids were formed by the small equiaxed α phase with interleaved lamellar α phase inside, and β phase was distributed at the boundary of α phase. The results calculated by flow stress model of Ti6Al4V alloy in α+β two-phase region was in good agreement with the experimental results, and the flow stress model had high accuracy.