Abstract: The uniaxial tensile property and cyclic plastic deformation behavior of U75VG rail steel were studied by uniaxial tensile tests and cyclic plastic deformation tests at different temperatures (25,300,600℃). The results show that the U75VG rail steel presented initial cyclic softening characteristics under strain-controlled cyclic load at 25℃, and initial cyclic hardening characteristics at 300℃ by the dynamic strain ageing. The dynamic strain ageing effect disappeared and the cyclic softening feature was obvious at 600℃. The U75VG rail steel exhibited obvious ratcheting behavior characteristics under stress-controlled cyclic load at different temperatures. The ratcheting strain rate increased with the increase of mean stress or stress amplitude. The ratcheting strain rate was close to the stable state at 300℃, and increased rapidly with the increase of mean stress or stress amplitude at 600℃.