Abstract: The fretting wear test of new type 25CrNi2MoV steel for high-speed and heavy-duty drive shaft was carried out by fretting friction and wear tester under dry friction condition. The fretting wear property of the steel under different loads (50-200 N) and frequencies (15-30 Hz) was studied. The results show that the average friction coefficient of 25CrNi2MoV steel decreased from 0.766 to 0.661, and the wear volume increased from 19.65×10^-3 mm³ to 75.83×10^-3 mm³ when the load increased from 50 N to 200 N at the frequency of 20 Hz. Under the load of 30 N, when the frequency increased from 15 Hz to 30 Hz, the average friction coefficient increased from 0.790 to 0.905, and the wear volume increased from 11.43×10^-3 mm³ to 23.88×10^-3 mm³. Under different test parameters, the oxidation and ploughing appeared on the wear surface of 25CrNi2MoV steel, indicating that the wear mechanism contained oxidation wear and abrasive wear. The wear mechanism of 25CrNi2MoV steel was mainly adhesive wear under the load of 50,100 N, and was fatigue wear under the load of 150, 200 N at the frequency of 20 Hz. Under the load of 30 N, the wear mechanism was mainly abrasive wear at the frequency of 15, 25 Hz, and was mainly fatigue wear at the frequency of 30 Hz.