Abstract: Damage characteristics such as cracks and pores on fracture and longitudinal slice of GH4169 alloy specimens were analyzed by scanning electron microscopy, after being tested under different creep-fatigue conditions (strain ratios of -1, 0, -∞; total strain ranges of 1.2%, 1.6%, 2.0%;tensile strain retention times of 60, 120, 300, 1 800, 5 400 s). The relationship between microscopic damage characteristics and experimental condition was explored. By electron backscatter diffraction technique, the initiation position and development process of the pores and cracks were qualitatively described, the creep damage was located and the damage mechanism of the alloy under the interaction of creep and fatigue was analyzed. The results show that the total strain range and the tensile strain retention time were inversely proportional to the specimen life, and were proportional to the crack source number and the number and size of cracks and pores on the longitudinal slice. The strain ratio had no significant effect on the specimen life. The creep damage accumulation of the specimen increased with the tensile strain retention time.