Abstract: A self-designed stiffness softening shear test system was used to conduct shear test on isotropic magnetorheological elastomers (MRE), and the force-displacement hysteresis loop under different current, frequency, and displacement amplitude conditions was obtained. The dynamic mechanical characteristics of MRE was described by Bouc-Wen model, and the accuracy of the model was verified on the basis of test data. The influence law of test parameters on macroproperties of MRE was analyzed. The results show that the fitting results of Bouc-Wen model were in good agreement with the test data, and the root mean square error of shear force was less than 0.95, indicating that the model could well describe the stiffness softening and damping characteristics of MRE. When the forward current was applied, the stiffness of MRE decreased linearly with increasing current, and increased with frequency; the effect degree of current or frequency on the stiffness was weakened when the current was greater than 0.66 A or frequency was greater than 1 Hz. The damping of MRE decreased with increasing current, and decreased exponentially with increasing frequency; the damping tended to be stable when the current was greater than 0.66 A or frequency was 2.0 Hz.