RGD功能化HA/PLLA多孔复合材料的制备和表征

Preparation and Characterization of RGD-HA/ PLLA Porous Composite

摘要: 通过碳二亚胺法将精氨酸-甘氨酸-天冬氨酸(RGD)接枝到羟基磷灰石(HA)颗粒表面, 增强HA颗粒识别细胞的功能, 然后将其均匀地分布于左旋聚乳酸 (PLLA) 中制备多孔复合材料, 分别用XPS和SEM等对HA颗粒和多孔复合材料进行了表征。结果表明: RGD成功地接枝到了HA颗粒表面, 多孔复合材料中RGD功能化的HA颗粒是纳米尺寸的, 且分布均匀; RGD功能化HA/PLLA多孔复合材料的细胞粘附率从普通HA/PLLA多孔复合材料的37.21%提高到了69.11%。

Abstract: RGD was grafted on HA particle surface by carbodiimide method to increase the cell recognizing function of HA particles, and then the particles were homogenously distributed in PLLA to prepare porous composite. HA particles and porous composite were characterized by XPS and SEM. The results show that RGD was successfully grafted on HA particle surface, RGD-HA particles were nano-size and homogenously distributed in the porous composite. Cell adhesive ratio of RGD-HA/PLLA porous composite increased from 37.21% of ordinary PLLA/HA to 69.11%.